PP-056 Monoclonal antibody-based ELISA versus commercial Fast Dot-ELISA technique in the diagnosis of human schistosomiasis and fascioliasis
نویسندگان
چکیده
منابع مشابه
A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial
capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...
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Dot-ELISA, a technique that shares the same principles as the enzyme immunoassay, is useful for detection of anti-Schistosoma mansoni antibodies in the sera of patients with Schistosoma mansoni infections. The antigens were fixed to the nitrocellulose strips, blocked with 1% bovine serum albumin in 0.05% Tween 20. Patient sera (40) and normal laboratory personnel sera (9) were applied to the sh...
متن کاملComparison of Dot-ELISA and Sandwich ELISA Diagnostic Tests in Detection of Human Hydatidosis
Hydatidosis, caused by the larval stage of Echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. As its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. Sera (250 samples) were collected from 90 cases of hydatidosis pro...
متن کاملDot ELISA for antibody detection in giardiasis.
Giardia lamblia is a frequent pathogen in persistent diarrhoea. Both humoral and cellular immune responses are important in clearing the parasite and providing immunity. A rapid sensitive and specific serological test like dot ELISA could be useful for early detection of Giardia specific serum antibodies for routine diagnosis. This study reports the use of dot ELISA for the detection of Giardia...
متن کاملA Monoclonal–Monoclonal Antibody Based Capture ELISA for Abrin
Abrin, one of the most highly potent toxins in the world, is derived from the plant, Abrus precatorius. Because of its high toxicity, it poses potential bioterror risks. Therefore, a need exists for new reagents and technologies that would be able to rapidly detect abrin contamination as well as lead to new therapeutics. We report here a group of abrin-specific monoclonal antibodies (mAbs) that...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2010
ISSN: 1201-9712
DOI: 10.1016/s1201-9712(10)60124-5